RasGRP1 signaling is required for Vγ2+ thymocyte c-Maf expression and γδT17 lineage programming.

18 Analysis of Nur77 GFP mice revealed a clear trend of reduced GFP expression correlating with increased c-Maf expression, and immature Vγ2 + thymocytes that were RORγt + expressed the lowest amount of GFP, suggesting c-Maf + and RORγt + cells received the weakest TCR signals ( Fig. 7D ).

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Analysis of Rag2pGFP mice found no significant difference in GFP intensity between immature c-Maf + and c-Maf - Vγ2 + thymocytes, but a nonsignificant trend towards immature c-Maf + RORγt + Vγ2 + thymocytes expressing GFP less intensely ( Fig. 7C ).

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