For the dose-dependent analysis ( Figure 4C ), RpHluorin2-expressing cells treated with JTC-801 at concentrations of 10, 20, and 40 μM for 6 h showed a clear trend of decreasing RpHluorin2 fluorescence with increasing JTC-801 concentration.
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Establishment of RpHluorin2-expressing cell and its application in monitoring JTC-801-induced alkaliptosis via multi-dimensional fluorescence detection approaches.
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