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A Standard Operating Procedure for Protein Extraction From Abdominal Aortic Aneurysm Tissue: Enhancing Proteomics Applications.

Proteomics Clin Appl · 2026 · PMC12743587 · PMID 41237014

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Regarding the effect of bead quantity on protein extraction efficiency, although the observed differences did not reach statistical significance, an improvement in protein yield was noted when using a bead quantity 30 times the tissue mass (4.14 ± 1.18%), compared to the other proportions tested (20 × , 3.75 ± 0.57%; 40 × , 3.83 ± 0.26%) (Figure 3A ).

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