Regarding the effect of bead quantity on protein extraction efficiency, although the observed differences did not reach statistical significance, an improvement in protein yield was noted when using a bead quantity 30 times the tissue mass (4.14 ± 1.18%), compared to the other proportions tested (20 × , 3.75 ± 0.57%; 40 × , 3.83 ± 0.26%) (Figure 3A ).
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A Standard Operating Procedure for Protein Extraction From Abdominal Aortic Aneurysm Tissue: Enhancing Proteomics Applications.
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