High-Efficiency l‑PEI-Based Transfection of ARPE-19 Cells Using a Multiparametric Approach and Automated Polyplex Formation with a 3D-Printed Microfluidic System.

Cell viabilities remained consistently ≥ 83%, although a significant trend was only observed between NP ratio 7.5 (and 10 only PP ma ) and 20, indicating slightly higher viability at higher N/P ratios, which could correlate with a reduction in the proportion of high producers ( Figure S11 ).

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