Bottom-Up Proteomics Under Acidic Conditions Using Protease Type XIII From Aspergillus saitoi.

Additionally, the average length of the identified peptides showed a decreasing trend with increasing digestion time and temperature ( Fig. 3 B and Supplemental Fig.

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In reversed-phase liquid chromatography separation, the retention time range showed a trend toward longer retention times at pH values of 2.5 or lower, but remained nearly constant at pH 2.5 or higher, differing from the peptide length pattern and exhibiting a retention time range similar to that of trypsin-digested peptides ( Fig. 1 C ).

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