Integrated genomic and transcriptomic Insights into methanol tolerance mechanisms in Methylobacterium extorquens AM1, identifying key targets for strain engineering.

Notably, Am01 exhibited the most substantial improvement in specific growth rate and was highly significant ( p -value < 0.01).

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Although the growth improvements conferred by the single metY mutations did not reach statistical significance in this study, a consistent trend of enhanced fitness compared to the wild-type was observed (Fig. 5 B, 5D).

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