Sorted SCβ-cells also showed a trend of increased connecting (C)-peptide secretion in response to glucose stimulation indicating their enrichment for SCβ-cells.
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Truncated CD19 as a selection marker for the isolation of stem cell-derived β-cells.
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The recovery rate (see ‘Magnetic-activated cell sorting for CD49a and CD19’ section in the Materials and Methods for the calculation method), showed a decreasing trend as the number of column enrichment steps increased ( Fig. 3C ).