Red light-driven enhancement of calycosin biosynthesis in Astragalus membranaceus via functional characterization of AmI3'H.

Real-time PCR analysis of gene expression levels revealed that, interestingly, genes from AmPAL to AmCHR did not show a significant trend (Fig. 4 a-d), but starting with the AmCHS gene, all genes including AmCHI , AmIFS , AmIOMT , and AmI3’H exhibited the highest expression levels in the red light-treated group (Fig. 4 e-i).

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The expression pattern of the AmI3’H gene in leaves co-infiltrated with formononetin and AmI3’H was interesting, showing a decreasing trend in AmI3’H gene expression as the harvest time was delayed (from 24 to 72 h) (Fig. 7 h).

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