approached significanceP = 0.086
Moreover, the polynomial contrast analysis indicated a significant linear ( P < 0.05), cubic ( P < 0.001) and quartic ( P < 0.001) effects in the relationship between treatment and DMD, whilst the negative quadratic trend approached significance ( P = 0.086), suggesting a highly non-linear relationship characterized by alternating increase and decrease of DMD across the different AcM levels with a pick on T3.