Host range and antibiotic resistance dissemination are shaped by distinct survival strategies of conjugative plasmids.

Consequently, while a trend toward mutual exclusion was robustly observed, it did not reach statistical significance owing to the predominance of “None” plasmids ( n = 10 716), which mainly originate from taxa outside of Enterobacterales (only 393 plasmids out of 10 716 “None” plasmids originate from Enterobacterales).

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It indicates that, both for individual ARGs and as an overall trend, the timing of ARG acquisition on H-NS plasmids precedes that on PsiB plasmids.

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