Gender had no significant effect on DA, DOPAC levels or DOPAC/DA ratio (ANOVA tables in Supplement 9 ). In both KO and CTRL mice, atropine-treated eyes showed slightly increased levels of DA and DOPAC 2 h after a single application compared with saline-treated eyes, but this difference did not reach statistical significance (DA: KO, atropine: 158.2 ± 6.3, saline: 148.5 ± 5.8; CTRL, atropine: 122.3 ± 7.0, saline: 116.9 ± 6.0, simple main effect treatment p = 0.29; DOPAC: KO, atropine: 122.5 ± 10.8, saline: 104.8 ± 12.3; CTRL, atropine: 106.2 ± 11.8, S: 97.1 ± 10.8, simple main effect treatment p = 0.27; Figure 4 ).
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