Aas + mutant strain showed a trend of reduced ppGpp accumulation at 3 h and, more notably, failed to sustain the elevated level seen in the WT at later time points, consistent with acyl-ACP synthetase restoring acyl-ACP as imported FAs accumulated overtime ( Figure 3D ) ( Jackowski et al., 1994 ).
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(p)ppGpp mediates persister formation in <i>Escherichia coli</i> during glucose to fatty acid shift.
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