a strong trendp = 0.059
As shown in Figure 6 B , when looking at the total number of cells expressing DeltaFosB in the LC, the two-way ANOVA showed a strong trend (n.s.) for an effect of alcohol, which was again independent from sex (Ethanol: F (1,28) = 3.87, p = 0.059, n.s.; Ethanol × Sex: F (1,28) = 0.69, p = 0.41, n.s.); when sexes were pooled together, IA2BC overall caused a 35.6% increase in LC cells expressing DeltaFosB (Student's t test: t (30) = 2.09, p ≤ 0.05).