Copy number and sequence variation in ribosomal DNA and the transposon, Pokey, in mutation accumulation lines of Daphnia obtusa.

Regression analysis revealed a highly significant relationship between 28S and 18S copy numbers ( R 2 = 0.996; P < 0.00001; Supplementary Fig. 1 in Supplementary File 3 ), with a regression coefficient close to the expected value of 1 (1.02).

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There was a clear trend of lower absolute change rates at longer intervals, with a mean rate of about 12 copies at an interval of 5 generations, 7 copies at an interval of 10 generations, and 5 copies at an interval of 15 generations.

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