Optimization of DADA2 in QIIME2 for improving fidelity in 16S rRNA V4 amplicon data analysis.

Biol Methods Protoc · 2026 · PMC12906231 · PMID 41696351

Europe PMC DOI

hedged sentences

0.0010

closest p · 0.0× alpha

0.0010

boldest claim

The sentences

highly significantP = 0.001actually significant

5B–D ) demonstrated the strongest and most stable group separations, with R 2 values around 0.452–0.453 and highly significant P = 0.001.

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a clear trendno p-value reported

By systematically comparing the microbial compositions derived from different truncation thresholds (trunc170–200) against a mock community, we observed a clear trend of increasing concordance with theoretical profiles as truncation length increased.

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