MicroRNA profiles in plasma-derived extracellular vesicles across the human lifespan.

Notably, miR-222-3p and miR-654-5p showed highly significant downregulation (adjusted p < 0.01), suggesting strong age-associated regulatory shifts.

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When stratified by sex, EV concentration showed a positive association with fast walking speed in both females ( r = 0.465, p = 0.031) and males ( r = 0.467, p = 0.039), and a trend toward significance in the chair test in males (r = 0.273, p = 0.144).

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CD14 and CD69 showed a trend toward an increase in the older group, though these differences should be interpreted cautiously, given the pooled nature of the samples.

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