Notably, the ∆rstA single reporter strain showed a trend toward forming more curved cells relative to the WT single reporter strain, similar to analyses of the ∆ rstA dual reporter strain to WT and ∆ tcdR dual reporter strains (Supplementary Fig. 8b ).
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In situ visualization of Clostridioides difficile phenotypic heterogeneity and single-cell morphology during gut infection.
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