Acute testicular hyperthermia leads to rapid loss of global piRNA and a concurrent increase in LINE1 activity within heat sensitive male germ cells.

Quantification of the piRNA band relative to total RNA species showed a trend to reduction in isolated populations of round spermatids (Fig. 5 e) and a significant drop in spermatocytes (Fig. 5 f).

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Results Analysis of sequencing data revealed that 93% of differentially expressed transcripts were upregulated following heat stress, with a notable trend to upregulation of transposable elements.

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