showed a trendP = 0.065
Hepatic αKlotho expression showed a trend towards higher levels in LSL than in LB hens (P = 0.065; Fig. 2 A) with a significant difference in the MI0 group (P = 0.024; Fig. 2 A), while tibial (P < 0.001; Fig. 2 B) and renal (P < 0.001; Fig. 2 C) αKlotho expression were significantly higher in LB hens than in LSL hens.