Barely Significant
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In ovo sexing and genotyping using PCR techniques: a contribution to the 3R principles in chicken breeding.

Sci Rep · 2026 · PMC12929762 · PMID 41723204

Europe PMCDOI
2
hedged sentences
0.0001
closest p · 0.0× alpha
0.0850
boldest claim

The sentences

highly significantP = 0.0001actually significant
A highly significant positive trend in hatchability was observed for the brown layer line ( P = 0.0001), with hatching rates increasing from 52% at ED4 to 87% at ED7.

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did not reach statistical significanceP = 0.085so close (0.05 < p ≤ 0.1)
non-sampled/non-punctured: 6%, n = 13/209) also did not reach statistical significance ( P = 0.085; OR = 1.89, 95% CI: 0.90–4.17). 215 samples were used to validate the accuracy of sexing and genotyping from allantoic fluid DNA subjected to both endpoint and multiplex PCRs with gel electrophoresis and fluorescence-based KASP assays.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.