Differentiation of mesenchymal stem/stromal cells into CD45<sup>+</sup> macrophage-like cells: expanding insights into MSC plasticity.

Flow cytometry analysis showed a clear trend of decreased CD45 − population in the M-CSF injected group ( p = 0.057) ( Figure 5 C), with a significant decrease of the PαS cells within the CD45 − population ( Figure 5 D).

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While the exact role of PDGFRα in macrophage function and behavior remains underexplored, its expression may not be significant in macrophages. 61 Previous reports showed that CD49 + MSCs with CFU-F capability exhibited a CD34 + CD45 med/low phenotype after isolation, which was substantially downregulated during culture. 52 Another recent study found “distinct CD45 − B-lymphoid and erythroid progenitor populations, whose activity was enhanced by BM stromal cells” within CD45 − CD31 − Ter119 − cells.

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