Gpr3 knockdown significantly suppressed the induction of Syn1 and Syn3 at 96 h, while Syn2 expression showed a downward trend that did not reach statistical significance ( Figures 7 A–7C).
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GPR3 is an immediate-early gene-like GPCR regulating CREB-dependent neuronal differentiation.
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The bidirectional NET-CAGE signals at both ends of the Gpr3 promoter showed a decreasing trend at 2 h after stimulation but increased again at 24 h ( Figure 3 ), mirroring the biphasic expression pattern of Gpr3 mRNA ( Figure 1 B).