Microbial succession and machine learning for postmortem interval estimation in bromadiolone-poisoned cadavers.

Statistical testing confirmed that these site-dependent differences were highly significant, for example in Shannon diversity (3.76 ± 0.55 vs. 1.88 ± 0.93, p < 0.001) and observed richness (320 ± 72 vs. 119 ± 194 ASVs, p < 0.001) (Fig.

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In the bromadiolone-treated group, Firmicutes increased sharply and became the predominant phylum, while Proteobacteria showed a trend of increasing followed by a decline over the decomposition period, indicating substantial restructuring of the anal microbial community under toxicological stress.

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