Factorial Design-Driven Optimization of Zein-Chitosan Nanoparticles for Oral Delivery of Silibinin.

A clear trend of increasing particle size is observed with higher concentrations of both zein and chitosan ( Figure a).

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The Pareto chart ( Figure c) identified the O:W ratio as the most influential factor on zeta potential, with a highly significant negative effect (−6.05).

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