Comparative Evaluation of Two Image-Analysis Software Platforms for Microfluidic Assessment of Red Blood Cell Deformability in Chronic Lymphocytic Leukemia.

Statistically significant differences ( p < 0.05, Mann–Whitney U test) were observed at intermediate shear rate levels corresponding to 268, 357, and 446 s −1 , whereas differences at the lowest and highest shear rates did not reach statistical significance.

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The mixed-effects analysis revealed a highly significant group effect for both analytical pipelines, confirming that RBCs from untreated CLL patients exhibit markedly reduced deformability compared with healthy donors across the tested shear range ( Table 3 ).

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