Histidine phosphorylation of NME1 regulates the Hippo pathway via the ARHGAP17-CDC42-cytoskeleton axis.

Western blot analysis revealed that overexpression of CDC42-G12V resulted in a modest reduction in p-YAP1 (Ser127) levels, although the change did not reach statistical significance—likely due to variability in transfection efficiency ( Fig. 5B and 5C ).

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