Genome-wide identification of the histone gene family in foxtail millet (Setaria italica) and analysis of its expression pattern in response to abiotic stress.

The enrichment analysis revealed that bZIP transcription factors were the most significantly overrepresented family predicted to bind histone gene promoters, with multiple members (e.g., Seita.1G043800) showing highly significant p -values ( p < 0.001) (Supplementary Table S7).

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The results revealed that although some histone genes, such as SiH2A.5 , SiH2A.11 , SiH2B.8 , SiH3.7 , SiH3.12 , and SiH4.4 , tended to be up-regulated, the changes did not reach statistical significance (Supplementary Figure S8D).

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