Dynamic regulation of secondary metabolites and agarwood aroma compounds in <i>Aquilaria sinensis</i> by the endophytic fungus NSZJ-CX-22 revealed through metabolomics and GC-MS.

In contrast, cluster 6 showed a trend of “increase from 0d to 7d and decrease from 7d to 28d”, reflecting a dynamic balance between metabolic activation in the early cultivation stage and metabolic inhibition in the later stage.

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In-depth analysis based on the culture time-dependent variation data of secondary metabolites presented in Table 6 revealed that sesquiterpenoids (including those with agarwood-characteristic skeletons) exhibited an overall trend of “increase–decrease–recovery”.

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