EV Tg 330 kDa normalized to CD63 showed an increasing trend according to a differentiation score of the lesion (calculated according to histology and aggressiveness) ( Supplementary Figure S3 ; Spearman’s rho = 0.430, p = 0.014).
It is interesting to note that free serum Tg levels did not correlate with EV Tg levels, except in the case of recurrent cases where a positive trend was observed; however, the very small sample size limits the interpretation of this result.
Levels of thyroglobulin bands at 330 kDa (EV Tg 330 kDa/CD63) and 200 kDa (EV Tg 200 kDa/CD63) showed a trend of higher expression in the malignant group; however, only levels of EV Tg 330 kDa/CD63 were significantly different between benign and malignant samples (Student’s t -test, p = 0.020; Cohen’s d = 0.848, 95% CI [−9.786, −0.909]; Figure 5 B). 2.4.