A clear trend is the coupling of RPA with next-generation signal amplification technologies—CRISPR/Cas12a or Argonaute—to push sensitivity down to single-copy or sub-copy levels and to enable multiplexed, visual detection in the field for viruses such as SDDV, LMBV, ISKNV and MRV or for EHP in shrimp supply chains [ 113 , 118 , 119 , 120 ].
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Progress of Rapid Detection Technology for Aquatic Microorganisms: A Comprehensive Review.
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