rected intraportal infusion of autologous CD31 + primary LSECs, isolated from each pig's liver biopsy and briefly expanded ex vivo, proved technically feasible and safe, and resulted in cell persistence without portal vein thrombosis. 48 Although reductions in fibrosis and biliary proliferation did not reach statistical significance, this study established a translational platform for primary LSEC transplantation in large animals. 48 Collectively, these studies demonstrate that primary LSECs can restore sinusoidal architecture, correct endothelial-dependent metabolic defects, and improve outcomes in both acute and chronic liver injury.
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