Contrasting temporal dynamics of fluorescence and photoacoustic signals from Cetuximab-IRDye800 conjugate in EGFR-overexpressing tumors.

At 705 nm, the overall temporal trajectory across all time points was significant (χ 2 = 13.20, P = 0.040, n = 6 animals), and the decline phase from 6 to 24 hours was highly significant (χ 2 = 12.56, P = 0.0014, n = 6 animals), with the 6- to 24-hour decrease confirmed by paired Wilcoxon test ( P = 0.031) and paired t test ( P = 0.003) (table S1).

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At 780 nm, the same temporal trends were observed but did not reach statistical significance at the animal level due to the limited sample size ( n = 5 to 9) (table S2).

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