Engineered T7 RNA polymerase to improve mRNA capping efficiency and reduce dsRNA generation during <i>in vitro</i> transcription.

Although we expected significant differences in Cas9 expression under the 0.5 mM cap condition based on the in vitro data, the R632N group showed only a trend toward increased Cas protein expression compared to WT T7 RNAP, and this difference did not reach statistical significance ( Figure 7 A).

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