Characterizing the metabolomes of microglia, astrocytes and neurons in ageing and Alzheimer's brains.

Among these, betaine, GSH and l -citrulline were significantly reduced, whereas choline and γ-aminobutyric acid exhibited downward trends that did not reach statistical significance (Extended Data Fig. 7f ).

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Consistent with our untargeted findings, GSH levels in 5xFAD microglia showed a decreasing trend relative to controls, although the difference did not reach statistical significance ( P = 0.080), probably owing to variability within groups (Supplementary Fig. 8a,b ).

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