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Free energy relationship analysis for temperature dependence of hydride kinetic isotope effects of NADH/NAD<sup>+</sup> model reactions: implication for barrier compression by enzyme dynamics.

Chem Sci · 2026 · PMC13181604 · PMID 42157898

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a clear trendno p-value reported
Although the fit is poor ( R 2 = 0.748), it reveals a clear trend that the reaction rate decreases as Δ G ° becomes less negative within the exergonic region and continues to decrease as the reactions become endergonic.

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