A Synaptogenesis-Associated Histomorphologic Signature from H&E Whole-Slide Images Predicts Glioma Prognosis and Identifies <i>EFNB2</i>-Positive Malignant Cells as a Candidate Neuro-Glioma Communication Hub.

Samples with higher malignant-cell EFNB2 expression showed a similar directional trend toward increased suppressive myeloid proportions; however, this difference did not reach statistical significance (Wilcoxon p = 0.144; Supplementary Figure S3 ).

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Consistently, comparison of continuous EFNB2 expression across the six WHO categories revealed a highly significant overall difference, with IDH -wildtype GBM showing the highest EFNB2 expression, whereas IDH -mutant astrocytomas and oligodendrogliomas generally exhibited lower levels ( Figure 5 C).

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