Perinatal mAChR-mediated activation of cortical subplate neurons elicits network activity driving basket cell differentiation.

At DIV 15 ( Figures 10E – H ), the AcD cells had a clear trend toward longer axons ( Figure 10F ), indicating that the significant increase of total axonal length at DIV 15 ( Figure 9G ) is mainly driven by AcD cells.

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The increase emerged at DIV 10 concurrent with the enhanced expression of VGAT-1 and Syt-2 and became highly significant at DIV 15, the major period of basket cell axon differentiation in the visual cortex.

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