When cells were seeded at a density of 200 cells/well in ultra-low-attachment 96-well plates, the shNT group formed significantly larger spheroids, and the total number of spheroids formed (diameter >100 μm) was notably higher, whereas the shCTSZ group showed a trend toward forming smaller spheroids.
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CTSZ-dependent anoikis resistance enhances malignant characters of glioblastoma via NF-κB signaling.
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