In the subgroup comparison between the high LDL-C and CAD subgroups, a highly significant difference was observed for LDL-C ( P = 5.8×10 -158 ) because the high LDL-C subgroup was originally defined using LDL-C levels.
This was particularly pronounced in the scatter plot ( Fig.3E ) within the high LDL-C range (>150 mg/dL); however, the influence of non-synonymous rare variants was also observed in the lower ranges (e.g., 130–150 mg/dL). DNA Methylation Changes due to Environmental Exposure and Correlation Structure Nominally significant ( P <0.05) changes in DNA methylation with aging and statin administration were observed at some of the CpG sites examined, suggesting that these could be biomarkers of environmental exposure; for example, at SC4MOL cg05119988, the DNA methylation level decreased with statin therapy ( Supplementary Fig.4E ) , mirroring the increase in DNA methylation levels observed with elevated LDL-C levels ( Fig.4A and Supplementary Table 4D ) .