Single-cell multi-omics analysis decodes molecular characteristics of sheep oocyte fate <i>in vivo</i> maturation.

Additionally, we identified key genes such as cyclin D2 ( CCND2 ), crucial for the G1/S transition in the cell cycle and highly significant during follicular development [ 50 , 51 ]; centromere protein K ( CENPK ), essential for vertebrate centromere assembly and chromosome segregation [ 52 ]; and GATA binding protein 6 ( GATA6 ), a critical transcriptional regulatory factor, playing a key role in ovarian follicle development [ 53 ], gradually upregulated during the developmental fate towards MII-stage ( Fig. 2c ).

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A moderately higher ratio was observed in the ET-1 group compared to the other groups, but this difference did not reach statistical significance ( Fig. 6o , p ). 4.

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