Genistein Pretreatment Attenuates Ovalbumin-Induced Food Allergy in Mice with Intestinal Barrier Preservation and Modulation of Gut Microbiota and Metabolites.

Gen pretreatment prevented this decrease, with splenic CD4 + Foxp3 + cell proportions in the Gen-pretreated groups comparable to those in the OVA group; however, these differences did not reach statistical significance ( Figure 2 D).

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In addition, diarrhea severity and incidence showed a decreasing trend in Gen-pretreated groups compared with the OVA group, with the most apparent reduction observed in the H-Gen group ( Figure 1 D,E).

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