Posterior shift of Shh-Fgf signaling in axolotl limb regeneration drives sequential digit formation.

The results showed that Shh expression at MDS was not significantly reduced compared with EDS ( Figure 1 P), whereas Fgf8 showed a decreasing trend ( p = 0.051) ( Figure 1 Q).

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However, these effects were weaker than those observed with intact MDS mesenchyme grafts and did not reach statistical significance compared with controls.

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