We examined the expression levels of HDA6 targets, including FLC and MAF2 , 3 , 4 , and 5 , by qPCR, observing a clear trend toward higher expression of MAF4 gene in the mbd4-2 hda6 double mutant ( Figure 5 D).
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The chromatin reader MBD4 regulates temperature- and flowering-responsive transcription by recognizing histone arginine methylation.
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