A multiplex hextuple luciferase assay for advancing cellular signaling analysis in plant cells.

Although the RFLuc signal was relatively constant across all mixtures, the slope of the curve showed a negative trend from left to right (i.e., at higher dilutions); however, the slope was not significantly different from zero ( Figure 4 B).

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The regression curve for constant RLuc ( Figure 4 H) showed a positive trend, with the signal intensity increasing by up to of 80% in the mixtures where NLuc was present in increasing proportion, indicating signal leakage between these two luciferases in the order of > 10 6 RLU/s.

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