Palmitoyltransferase DHHC7 mediates protein palmitoylation and is essential for sperm function through the modulation of [Ca<sup>2+</sup>]<sub>i</sub> and ROS signaling.

Both Manders’ tM1 ( Figure 4F ) and tM2 ( Figure 4G ) in the DHHC7 groups were markedly elevated above 0.5 relative to control, with all differences highly significant (P < 0.0001).

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The differences observed between Blank and IgG control groups did not reach statistical significance (P > 0.05) and are consistent with normal biological variability between sperm preparations.

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