Patient-derived tissue slice cultures from endoscopic biopsies as a translational ex vivo model for inflammatory bowel diseases.

Immunohistochemical analysis demonstrated stable CD68+ macrophage densities in both IBD and control tissue and a significant trend toward increased neutrophil infiltration in IBD samples.

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Quantitative analysis using linear mixed models with patient as random effect ( N = 12 IBD patients; n = 52 biopsies; m = 85 slice cultures) and FDR-adjusted P -values (Benjamini-Hochberg method, q < 0.05) revealed highly significant reductions ( q < 0.001) in 17 of 24 analytes, including key pro-inflammatory mediators (TNFα, IL-6, IL-8, IFNγ), chemotactic factors (CXCL5/ENA-78), growth factors (G-CSF, GM-CSF), and the anti-inflammatory cytokine IL-10.

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