Characterization of Fluorophore-Quencher Pairs for Distance-Dependent Molecular Sensing in Photoacoustic Imaging.

Conversely, Cy7.5 shows declining photoacoustic signals at both the contact quenching and fluorophore absorption peaks ( Figure S6 ), without demonstrating a clear trend based on distance, thereby making it a less suitable candidate for quenching or FRET-based sensor applications.

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