Dysregulation of the HSF1-Mediated UPRmt Pathway in Colonic Smooth Muscle Cells Drives Motility Dysfunction in Functional Constipation

RT-qPCR analysis confirmed that HSF1 and HSP60 mRNA levels were significantly increased following LV-HSF1-OE ( p < 0.001 and p < 0.05, respectively; Figure 9 D), whereas their reduction following LV-shHSF1 did not reach statistical significance at the mRNA level ( p = 0.08 and p = 0.17, respectively; Figure 9 D), despite robust protein knockdown; LONP1 and mtHSP70 mRNA levels showed trends consistent with their corresponding protein changes, although these differences did not reach statistical significance ( Figure 9 D).

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