Pleiotropic germline PTEN mutations influence gastrulation through dysregulated AKT activation.

We found that PTEN G132D/WT hiPSCs showed a trend of higher expression levels of PS markers, Brachyury, and MIXL1 than hiPSCs of other genotypes after 24-hour CHIR pretreatment (Supplementary Fig. 1b, c, e ).

also in 53,322 other papers

Western blotting detected a decreasing trend in protein levels of pluripotency marker OCT4 after CHIR pretreatment, suggesting that these primed cells still exhibit pluripotency characteristics as described elsewhere 28 ( p > 0.05) (Supplementary Fig. 1d ).

also in 47,180 other papers