Single-molecule kinetic exploration of functional sub-states in an evolving phosphotriesterase.

Nat Commun · 2026 · PMC13342112 · PMID 42067523

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a clear trendno p-value reported

The CV showed a clear trend when compared to the activity ratio ( k cat / K m ; PTE/AE): AE-specialized variants (PTE/AE < 10 −1 ) exhibited low CV 1H , while bifunctional variants (10 −1 < PTE/AE < 10 1 ), as well as PTE-specialized variants (PTE/AE > 10 1 ), displayed higher CV 1H values (Fig. 3C, D ).

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